Transcriptome Analysis Reveals Key Genes Involved in the Response of Pyrus betuleafolia to Drought and High-Temperature Stress

Transcriptome Analysis Reveals Key Genes Involved in the Response of Pyrus betuleafolia to Drought and High-Temperature Stress

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Drought and high-temperature stress are the main abiotic stresses that alone or simultaneously affect the yield and quality of pears worldwide.However, studies on the mechanisms MASCARA CLASSIC BLACK #1305 of drought or high-temperature resistance in pears remain elusive.Therefore, the molecular responses of Pyrus betuleafolia, the widely used rootstock in pear production, to drought and high temperatures require further study.Here, drought- or high-temperature-resistant seedlings were selected from many Pyrus betuleafolia seedlings.The leaf samples collected before and after drought or high-temperature treatment were used to perform RNA sequencing analysis.

For drought treatment, a total of 11,731 differentially expressed genes (DEGs) were identified, including 4444 drought-induced genes and 7287 drought-inhibited genes.Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these DEGs were more significantly enriched in plant hormone signal transduction, flavonoid biosynthesis, and glutathione metabolism.For high-temperature treatment, 9639 DEGs were identified, including 5493 significantly upregulated genes and 4146 significantly downregulated genes due to high-temperature stress.KEGG analysis showed that brassinosteroid biosynthesis, arginine metabolism, and proline metabolism were the most enriched pathways for high-temperature response.Meanwhile, the common genes that respond Water Bottles to both drought and high-temperature stress were subsequently identified, with a focus on responsive transcription factors, such as MYB, HSF, bZIP, and WRKY.

These results reveal potential genes that function in drought or high-temperature resistance.This study provides a theoretical basis and gene resources for the genetic improvement and molecular breeding of pears.